Wet Lab: Essential Techniques for Oocyte Collection and Incorporation of ICSI-Produced Embryos in an Equine Practice
Instructors: Ryan A. Ferris, Elaine Carnevale, Tom A. E. Stout
Friday, December 6
8:00 a.m. - 5:00 p.m.
Location: Colorado State University's Equine Reproduction Laboratory, Fort Collins, CO.
Requires an additional ticket: $950. You can register for this lab while registering for the Convention.
Participants will gain valuable hands on experience relative to ovarian positioning for follicular aspirations in live mares, techniques for transvaginal aspirations, identification and handling of oocytes, packaging of oocytes for shipment and receiving fresh or cryopreserved in vitro produced embryos. Participants must be familiar with equine ovarian palpation to participate.
Introduction: Welcome and Introduction to Techniques
Session 1 - Ovarian Positioning for Follicular Aspirations in Live Mares
Mare sedation and preparation will be reviewed and methods for follicle aspirations.
Participants will practice positioning the transvaginal probe and manipulating ovaries for follicle collections. Participants must be familiar with equine ovarian palpation to participate in this session.
Session 2 - Techniques for Transvaginal Aspirations
Mare tracts will be suspended to allow participants to study anatomical considerations and to perform procedures for transvaginal follicle aspirations.
Session 3 - Isolation and Handling of Oocytes
Participants will scrape ovarian follicles, search and handle oocytes.
Session 4 - Packaging of Oocytes for Shipment and Receiving Fresh or Cryopreserved Embryos
Participants will go through procedures and practice packaging ovaries and oocytes for shipment. Methods to handle fresh and cryopreserved ICSI-produced embryos will be included.
General Review - Reassemble and general questions
Participants will arrive at the Equine Reproduction Laboratory at 7:45 am and depart at 5:00 pm. Laboratory sessions will run concurrently to provide limited numbers of participants per session, individualized instruction, and promote hands-on learning.
AAEP/ISELP Wet Lab: Essential Techniques for the Ultrasound Exam of the Equine Stifle and Neck
Instructors: Myra F. Barrett, Erin Contino, Sherry A. Johnson, Elizabeth Acutt, A. Kent Allen, Russ Peterson, Roland Thaler, Cooper Williams, Jessica Morgan, Duncan Peters
Saturday, December 7
8:00 a.m. - Noon and 12:30 p.m. - 4:30 p.m.
Location: Colorado State University's C. Wayne McIlwraith Translational Medicine Institute, Fort Collins, CO.
Requires an additional ticket: $450 for AAEP or ISELP members/$500 for nonmembers.
This is a half-day lab being offered twice - AM session for 50 ppl and PM session for 50 ppl. You can register for this lab while registering for Convention.
The equine neck and stifle have complex soft tissue and bone anatomy that is often challenging to image. Attendees will explore the pertinent anatomy and correlative imaging. Clinical cases will be discussed with the instructors as well as approaches to diagnosing injuries and treating the equine athlete with clinicians who make a living doing just that. Different conditions that will be reviewed include disease processes of the stifle and neck and key desmopathies/enthesopathies. The demonstration will also include a neck ultrasound guided injection to familiarize practitioners with that technique.
The session will be spent in an intense exploration of ultrasonographic examination of the equine neck and stifle with specific goals. A group of talented clinicians will assist in the imaging session with small groups and plenty of time.
Transportation by bus to Fort Collins is included with registration.